Instructions for Barron Artificial Anterior Chamber

The Barron Artificial Anterior Chamber is comprised of three pieces: a base with a tissue pedestal, a tissue retainer and a locking ring. The base has two ports with silicone tubing, in-line pinch clamps, and female Luer Lok connectors. Either port may be used to inject or aspirate viscoelastic, balanced salt solution, or air beneath the donor cornea.

For best results, the outside diameter of the donor tissue
should be less than 18mm but not smaller than 14mm.
Suggestions for Use
  • Inject balanced salt solution into one of the tubing ports. Remove the excess from the well in the tissue pedestal.
  • Deposit a drop of viscoelastic material into the well of the tissue pedestal prior to placement of the donor tissue. Place the donor cornea on the tissue pedestal with the epithelium facing up.
  • With the donor tissue in place, carefully align the two holes in the tissue retainer with the two pegs projecting from the base and press down firmly.
  • Place the locking ring over the tissue retainer and turn clockwise until the ring is locked firmly in place.
  • Additional viscoelastic, BSS, or air may now be injected to obtain the desired pressure beneath the donor cornea. It is suggested to use one port for irrigation and the other for air. Both pinch clamps may be locked to maintain the desired pressure while the donor cornea is being cut.
  • For penetrating keratoplasty procedures, use a Barron Radial Vacuum Trephine for cutting the donor cornea.

Demonstration of Barron Artificial Anterior Chamber

An example of the use of the Barron Artificial Anterior Chamber for preparing donor cornea tissue for the DLEK procedure is shown in the following video prepared by Dr. M. Bowes Hamill (Baylor College of Medicine). The video also shows the Barron Vacuum Donor Punch being used to cut the donor button after the stroma has been split using the artificial anterior chamber. The last half of the video shows the insertion into the patient’s eye of the donor tissue.

DLEK Procedure using Barron Artificial Anterior Chamber and Barron Vacuum Donor Punch

Suggestions for Use – Steel Base

The accessory steel base was designed to be used with the Barron Artificial Anterior Chamber. The steel base has 4 sets of threads that willengage the bottom opening of the Anterior Chamber. The engagement is a press fit, and the threads are “self-tapping” so the user should not expect the Chamber to just simply screw onto the base.

  • Position the steel base on a firm flat surface, and hold it in one hand along the finger grips that are on the perimeter of the base.
  • Hold the base of the artificial anterior chamber in the other hand and place it squarely over the threaded pedestal of the steel base.
  • Push the artificial anterior chamber base down firmly and simultaneously twist it clockwise approximately 15-20 degrees.
  • The chamber base should be secured snugly in place. As noted above, the locking mechanism is a press fit, with the threads on the base making it easier to accomplish the press fit.
  • To remove the chamber from the steel base, simply hold the steel base in one hand and turn chamber base counterclockwise not expect the Chamber to just simply screw onto the base.

Example video of using the Barron Artificial Anterior Chamber steel base